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In vitro culture, PCR , and nested PCR for the detection of Theileria equi in horses submitted to exercise Arq. Bras. Med. Vet. Zootec.
Baldani,C.D.; Canola,P.A.; Neto,J.C.L.; Machado,R.Z..
This study compared the usefulness of in vitro culture, PCR, and nested PCR for the diagnosis of Theileria equi in horses submitted to stress during exercise. Blood samples from 15 apparently healthy horses, previously conditioned to a high-speed equine treadmill, were taken prior to and after exercise. The animals were divided into two experimental groups: 30-day training schedule (G1) and 90-day training schedule (G2). Statistical analysis was performed using a chi-square test and kappa statistic was used in order to assess agreement. No significant difference was observed between samples collected at resting or after exercise. In G1, merozoites of T. equi were detected in the blood smears of four horses before in vitro culture, whereas 14 samples were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eqüino; Theileria equi; Diagnóstico; Cultivo in vitro; PCR.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000300005
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Occurrence of congenital in Theileria equi Lusitano pure blood foals, diagnosed by RT-PCR MV&Z
Roncati, Neimar V.; Baccarin, Raquel Yvonne Arantes; Massoco, Cristina de Oliveira; Fernandes, Wilson Roberto.
In order to determine the occurrence of transplacentary transmission of Theileria equi in equine neonates, 50 foals of the Lusitano breed, both colts and fillies, along with their respective mothers were evaluated shortly after parturition. Total blood samples were collected from the mothers and the neonates within the first five hours after parturition for the detection of Theileria equi, through use of the RT-PCR technique. The detection kit based on the intercalating dye of DNA SYBERgreen was used. A total of 46% of the mares presented a positive result for Theileria equi and 54% presented negative ones, while 66% of the foals presented positive results, seeing as 73.9% of them were born from also positive mothers. The remaining 34% of foals presented...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Theileria equi; Babesia equi; Babesiosis; RT-PCR; Transplacentary transmission; Congenital Theileria equi; Bacteria equi; Babesiose; RT-PCR; Transmissão transplacentária; Congênita.
Ano: 2011 URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/386
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Seroprevalence of Babesia caballi and Theileria equi in horses and mules from Northern Thailand OAK
Kamyingkird, K.; Yangtara, S.; Desquesnes, M.; Cao, S.; Adjou Moumouni, P.K.; Jittapalapong, S.; Nimsupan, S.; Terkawi, M.A.; Masatani, T.; Nishikawa, Y.; Igarashi, I.; Xuan, X..
Palavras-chave: Babesia caballi; ELISA; IFAT; PCR; Theileria equi.
Ano: 2014 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4092
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